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Peptide separations by slab gel electrophoresis in pluronic F127 polymer liquid crystals
Authors:Rill Randolph L  Al-Sayah Mohammad A
Institution:Department of Chemistry and Biochemistry, College of Medicine, The Florida State University, Tallahassee, FL, USA. Randolph.Rill@med.fsu.edu
Abstract:Proteomics and peptidomics could benefit from simple methods for high-resolution separation of oligopeptides analogous to slab gel electrophoresis of proteins. Gels of Pluronic F127 copolymer surfactant were investigated as media for slab gel electrophoresis of oligopeptides using a trypsin digest of myoglobin. Concentrated solutions of Pluronic F127 are fluid at low temperatures (
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