Total chemical synthesis of biologically active vascular endothelial growth factor |
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| Authors: | Mandal Kalyaneswar Kent Stephen B H |
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| Institution: | Department of Biochemistry and Molecular Biology, Institute for Biophysical Dynamics, The University of Chicago, IL 60637, USA. |
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| Abstract: | Efficient access: the 204-residue covalent-dimer vascular endothelial growth factor with full mitogenic activity was prepared from three unprotected peptide segments by one-pot native chemical ligations. The covalent structure of the synthetic VEGF was confirmed by precise mass measurement, and the three-dimensional structure of the synthetic protein was determined by high-resolution X-ray crystallography. |
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| Keywords: | chemical protein synthesis growth factors mass spectrometry one‐pot ligation VEGF |
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