原子吸收光谱法测定花粉中的微量元素

采用中温灰化样品，6 mol·L-1 HNO3浸取灰化物，用LaCl3和CsCl消除相关干扰，应用标准曲线法在同一溶液中利用空气-乙炔火焰原子吸收光谱法测定花粉中Zn，Cd，Fe，Mn，Pb，Mg，Cu，K，Ca和Na共10种元素含量。结果表明，花粉含有丰富的Zn,Mg,K,Ca,Na以及Fe,Mn,Cu，有害元素Pb的含量很低，Cd未检出。以野霍香花粉为例，详细研究确定了合适的样品处理及消除干扰条件，考察了方法的准确度和精密度。在选定的条件下，花粉中各元素间相互不干扰，可在同一份制备液中进行10种元素的分别测定。方法的加标回收率在93.4%～112.0%之间，相对标准偏差在0.3%～2.6%之间(n=5)，测定结果准确可靠。

Determination of Trace Elements in Pollen by Using Atomic Absorption Spectrometry

After the middle temperature cinefaction sample was dipped in 6 mol·L-1 HNO3,the relevant disturbance was removed by LaCl3 and CsCl,and the contents of ten elements(Cu,Fe,Mn,Zn,Pb,Cd,Ca,Mg,K and Na) were determined by flame atomic absorption spectrometry in the same solution by standard curve method. The experimental result indicates that there are abundant Zn,Mn,K,Ca,Na,Fe,Mg and Cu in the pollen,while the content of Pb is very low,and there is no Cd. Using the wild ageratum pollen as the sample,the proper condition of sample disposing and how to remove disturbance were studied and determined in detail,and the accuracy and precision of the method were examined. As a result,different element in the pollen doesn′t interfere with each other under the chosen condition. These ten elements can be determined in the same preparation solution respectively. The recovery is between 93.4％ and 112.0％,the relative standard deviation is between 0.3% and 2.6％ (n=5). The result is accurate and reliable.