白芍紫外指纹图谱共有峰率和变异峰率双指标序列分析

采用共有峰率和变异峰率双指标序列分析法对不同产地、不同采集年份的白芍样品及相近植物赤芍、丹皮样品进行了定量评价。白芍等紫外指纹图谱双指标序列分析结果显示，相近产地的3份白芍B2，B3，B4具有最相近关系, 它们之间共有峰率大于70%，变异峰率都小于33.33%。而不同产地的白芍之间存在着较明显的差异，B1，B5与B2，B3，B4之间的共有峰率小于60%，与B6的共有峰率小于57%。同一产地不同年份的白芍B1，B5之间存在着较大的差异，它们之间的共有峰率仅为44.4%, 变异峰率高达100%。该法可以对至少2个样品进行定量评价，克服了多种中药鉴别方法只能进行真伪鉴别、产地鉴别的局限性，为中药质量的准确定量评价提供了一种新方法。

The Common and Variation Peak Ratio Dual Index Sequence Analysis on UV Fingerprint Spectra of Paeonia Lactiflora Pall.

Baishao and Chishao(Paeonia Lactiflora Pall.) and their close relative Danpi(Paeonia Suffruticosa Andr) samples were estimated quantitatively,based on their UV fingerprint spectra of the extracts obtained with chloroform,ethanol and water,by applying the common and variation peak ratio dual index sequence analysis method.The analytical results showed that the Baishao samples B2,B3 and B4 from the closest regions were the most similar samples.Their common peak ratios were larger than 70 percent and their variation peak ratios were less than 33.3 percent.However,there existed obvious differences among Baishao sample group 1(B1 and B5),group 2(B2,B3 and B4) and group 3(B6) from different regions.The common peak ratios among group 1(B1 and B5) and group 2(B2,B3 and B4) were lower than 60 percent,while those among group 1(B1 and B5) and group 3(B6) were less than 57 percent.The Baishao samples B1 and B5 from the same region collected in different years were of significant disparity,their common peak ratio was only 44.4 percent,but their variation peak ratios were larger than 100 percent.In fact,this method reaches the limitation of quantitative identification of herbs,and can distinguish at least two samples quantitatively.