大鼠死后肺脏FTIR光谱变化与死亡时间的关系

应用傅里叶变换红外(FTIR)光谱分析大鼠死后肺脏组织随死亡时间推移的化学降解过程，为法医死亡时间推断提供新的研究方法。结果表明，随着死亡时间的推移，大鼠肺脏组织FTIR光谱的主要吸收峰峰位没有明显变化，而其峰高有明显差异：(1)与核酸有关的1080，1241cm-1谱带的峰强呈下降趋势。(2)酰胺Ⅰ(I₁₆₄₇)，Ⅱ(I₁₅₄₁)峰强比随死亡时间推移下降。1338和1313cm-1处的吸收峰强略有升高。(3)1460， 1400，1170 cm-1的谱带没有明显变化趋势。(4)死亡72 h后，1120 cm-1处出现新的吸收峰，吸收强度随时间推移逐渐增高；这一结果表明FTIR光谱分析技术有望成为法医死亡时间推断的有效方法。

Study on Estimation of Postmortem Interval Using FTIR in Rat Lung

Fourier transform infrared spectroscopy (FTIR) was applied to study the decomposition process postmortem in rat lung tissue and provided a new method for the estimation of postmortem interval (PMI). The results demonstrated that, with the PMI increasing, the peak position of main absorbance bands showed no significant difference, but there was obvious variance of intensity: (1) The intensity of 1080 and 1241 cm(-1) related to nucleic acid tended to decrease. (2) The intensity ratio at Amide I (I1647) and II (I1541) decreased since death. The intensity of 1338 and 1313 cm(-1) increased lightly. (3) The intensity at 1460, 1400 and 1170 cm(-1) showed no significant difference. (4) A new band at 1120 cm(-1) appeared since 72 h after death and its intensity increased from 72 to 144 h postmortem. The authors' preliminary results suggest that it may be possible for FTIR to become an effective method to estimate the PMI in forensic practice.