| A STUDY ON TUMOR FORMATION OF SOYBEAN AND GENE TRANSFER |
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| 作者姓名： | 王连铮 尹光初 罗教芬 雷勃钧 王剑 姚振纯 李秀兰 邵启全 蒋兴邨 周泽其 |
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| 作者单位： | Heilongjiang Provincial Academy of Agricultural Sciences，Harbin Institute of Genetics，Academia Sinica，Beijing，Heilongjiang Provincial Academy of Agricultural Sciences，Harbin，Heilongjiang Provincial Academy of Agricultural Sciences，Harbin，Heilongjiang Provincial Academy of Agricultural Sciences，Harbin，Heilongjiang Provincial Academy of Agricultural Sciences，Harbin，Heilongjiang Provincial Academy of Agricultural Sciences，Harbin，Heilongjiang Provincial Academy of Agricultural Sciences，Harbin，Institute of Genetics，Academia Sinica，Beijing，Institute of Genetics，Academia Sinica，Beijing，Institute of Genetics，Academia Sinica，Beijing |
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| 摘 要： | From 15 strains of Agrobacterium tumefaciens 7 were selected as the best inductors oftumors on soybean and 94 tumor formation genotypes were screened from 1553 varieties andforms of Glycine soja, G. gracilis and G. max. The frequency of tumor formation was 6%.The bacteria-free calluses were obtained. The data of biochemical analysis verify that someof the above-mentioned calluses contained nopaline. This means that Ti-plasmid of Agrobac-terium tumefaciens can be used as a vector for the transfer of nopaline, a marker gene, intogenome of several species of soybean. In the soybean genome the transferred nopaline genewas integrated and expressed. The transformed gene can be stably integrated in the soybeangenome through vegetative propagation.
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