Synthesis of diketopyrrolopyrrole‐containing conjugated polyelectrolytes for naked‐eye detection of DNA |
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| Authors: | Shuimu Lin Shouping Liu Hanxun Zou Wennan Zeng Lingyun Wang Roger Beuerman Derong Cao |
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| Institution: | 1. Department of Chemistry, School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510640, China;2. Singapore Eye Research Institute, Singapore 168751, Singapore;3. State Key Laboratory of Physics and Chemistry of Luminescence, South China University of Technology, Guangzhou 510640, China |
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| Abstract: | Two water‐soluble cationic conjugated polyelectrolytes ( P1 and P2 ) containing diacetylene, diketopyrrolopyrrole (DPP), and fluorene units were synthesized with Glaser‐Hay coupling reaction as the key step. The narrow bandgap DPP units and the wide bandgap fluorene units in the cationic polyelectrolytes might form an energy donor‐acceptor molecule architectures, in which DPP units serve as an acceptor of the fluorescent resonance energy transfer. The addition of calf thymus DNA enhances the fluorescent resonance energy transfer from fluoreneethynylene segments to DPP units, which results in a sensitive color change from blue to red in the PL spectra and allows naked‐eye detection of DNA with low concentration. In addition, the detection of DNA with P1 and P2 is high selective because it is not interfered by common ions. © 2011 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem, 2011 |
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| Keywords: | cationic conjugated polyelectrolytes conjugated polymers CT DNA diketopyrrolopyrrole fluorescence fluorescence resonance energy transfer fluorescence sensors |
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