Determination of cobalt marker in cow ruminal fluid by EDXRF and SRTXRF |
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| Authors: | Eduardo de Almeida Paulo Rogério Massoni Amauri Antonio Menegário Laudi Cunha Leite Dante Pazzanese Duarte Lanna Virgílio Franco do Nascimento Filho |
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| Affiliation: | 1. Laboratório de Instrumenta??o Nuclear (LIN), Centro de Energia Nuclear na Agricultura (CENA), Universidade de S?o Paulo (USP), Avenida Centenário, no. 303, CEP 13400‐970, Piracicaba, SP, Brazil;2. Centro de Estudos Ambientais (CEA), Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), Av. 24‐A, no. 1515, CEP 13506‐900, Rio Claro, SP, Brazil;3. Laboratório de Nutri??o e Crescimento Animal (LNCA), Departamento de Zootecnia, Escola Superior de Agricultura “Luiz de Queiroz” (ESALQ), Universidade de S?o Paulo (USP), Av. Pádua Dias, no. 11, CEP 13418‐970, Piracicaba, SP, Brazil |
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| Abstract: | This article describes a comparison of conventional energy‐dispersive X‐ray fluorescence (EDXRF) and synchrotron radiation total‐reflection X‐ray fluorescence (SRTXRF) for Co determination in ruminal fluid from Holstein cow. This element is used as marker for animal nutrition studies. For EDXRF, 200 µl of the sample were dried on 6.35 µm Mylar film at 60 °C. The excitation was carried out using an X‐ray tube with Mo target and Zr filter operated at 30 kV/20 mA. For SRTXRF, 10 µl of the sample were pipetted on a Lucite carrier and dried at 60 °C. In both the techniques, Ga was used as internal standard and the acquisition time was 200 s. The trueness of both techniques was evaluated through the standard addition method, the recoveries obtained by SRTXRF and EDXRF were 76 and 99%, and the limits of detection, 13 and 240 µg l?1, respectively. Copyright © 2011 John Wiley & Sons, Ltd. |
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