反相高效液相法测定血浆及尿液中的异烟肼

 建立了血浆及尿液中异烟肼的高效液相快速测定方法 ,以满足临床药物分析和法医学鉴定的需要 ,提高对血浆及尿液中异烟肼浓度检测的准确性。以香草醛为衍生化试剂 ,将异烟肼经柱前衍生为异烟肼 香草醛腙 ,直接对处理后样品中的腙进行定性、定量分析。以在空白人体液样本中定量添加标准异烟肼的方法考察了样品的前处理方法、仪器条件、线性范围、精密度、回收率等 ,并对健康受试者血液中的异烟肼浓度进行了监测。结果表明 ,方法的线性范围为 0 2mg/L～ 1 2 0mg/L ;检测限为 0 2mg/L ;日内、日间精度均小于 4 0 % (n =5) ;回收率在96 3 %以上。

Determination of Isoniazid in Blood and Urine Samples by Reversed-Phase High Performance Liquid Chromatography

For the purpose of more accurate and rapid analysis of isoniazid in body fluid samples, a reversed phase high performance liquid chromatographic (HPLC) method was developed Vanillin, being as a derivatizing reagent, was added to the plasma and urine samples when they were pretreated Isoniazid and vanillin reacted to form isonicotinoyl hydrazone which was separated and detected The pretreatment method of sample, the linear range, the precision, and the recovery of isoniazid were investigated by using human's plasma and urine spiked with standard isoniazid The linear range was 0 2 mg/L-12 0 mg/L (for plasma, r =0 999?6; for urine, r =0 999?4) The detection limit was 0 2 mg/L The intra day and inter day precisions of assay for isoniazid were 2 3%-2 6% and 3 8%-4 0% ( n =5) for plasma, 1 3%-4 0% and 2 1%-3 9% ( n =5) for urine respectively The average recoveries of isoniazid were from 96 5% to 99 8% in plasma and from 96 3% to 99 4% in urine The HPLC method has been used to investigate the concentration of isoniazid in the volunteer's plasma The quantitative analysis of isoniazid in plasma was not interfered by impurities, the metabolites of isoniazid and the derivatizing reagent residue This analytical method for isoniazid is sensitive, rapid and convenient It is suitable for the analysis of toxics in forensic samples, the detection of drug concentration in clinical medicines and pharmacokinetic studies