| 奶粉中唾液酸的测定方法 |
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| 引用本文： | 黄华军,奚星林,吴宏中,李宪华.奶粉中唾液酸的测定方法[J].分析测试学报,2006,25(4):129-131. |
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| 作者姓名： | 黄华军 奚星林 吴宏中 李宪华 |
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| 作者单位： | 广州出入境检验检疫局,食品实验室,广东,广州,510623;广州出入境检验检疫局,食品实验室,广东,广州,510623;广州出入境检验检疫局,食品实验室,广东,广州,510623;广州出入境检验检疫局,食品实验室,广东,广州,510623 |
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| 摘 要： | 利用酸水解把奶粉中的唾液酸释放出来，通过聚丙烯酰胺凝胶除去奶粉中大量乳糖的干扰。利用Cu^2＋和Cr^3＋协同催化唾液酸与间苯二酚的显色，用乙酸丁酯-正丁醇萃取有色物质，在610nm处测定牛奶中唾液酸含量。方法的线性范围0～55．90mg／L，回收率为94％～96％。
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| 关 键 词： | 唾液酸 分光光度法 聚丙烯酰胺凝胶 奶粉 |
| 文章编号： | 1004-4957（2006）04-0129-03 |
| 收稿时间： | 2005-07-21 |
| 修稿时间： | 2005-10-08 |
Determination of Sialic Acid in Milk Powder |
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| HUANG Hua-jun,XI Xing-lin,WU Hong-zhong,LI Xian-hua.Determination of Sialic Acid in Milk Powder[J].Journal of Instrumental Analysis,2006,25(4):129-131. |
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| Authors: | HUANG Hua-jun XI Xing-lin WU Hong-zhong LI Xian-hua |
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| Abstract: | Based on the synergistical action of Cu~(2+) and Cr~(3+) to catalyze the color reaction of sialic acid with resorcinol,a spectrophotometric method for the determination of sialic acid in milk powder was developed.The sialic acid was released from the milk powder through acid hydrolysis,and the lactose matrix was removed by using the polyacrylamide gel column.The extracted colored compound was measured by a visible spectrophotometer at the wavelength of 610 nm.The linear range of the method was 0-55.90 mg/L,and the recovery was 94%-96%. |
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| Keywords: | Sialic acid Spectrophotometry Polyacrylamide gel Milk powder |
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