Comparative study of capillary zone electrophoresis and micellar electrokinetic chromatography for the separation of twelve aromatic sulphonate compounds |
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Authors: | M J Cugat F Borrull M Calull |
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Institution: | 1. Department de Química Analítica i Química Orgànica, Universitat Rovira i Virgili, P?a. Imperial Tarraco 1, 43005, Tarragona, Spain
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Abstract: | Summary Two modes of capillary electrophoresis (CE), capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography
(MEKC), were investigated for the separation of 12 aromatic sulphonate compounds. In CZE, although the voltage applied, the
buffer concentration and the pH were optimized for effective separation of the compounds studied, under the best conditions
four of the five amino compounds coeluted, as did naphthalene-1-sulphonic acid and naphthalene-2-sulphonic acid. In MEKC,
sodium dodecyl sulphate (SDS) and Brij 35 were chosen as the anionic and nonionic surfactants and the effect of the concentration
of micelles was examined. The effect of adding methanol as the organic modifier was also investigated with each of these micellar
systems. All the analytes, including the isomers, were completely separated by use of MEKC with Brij 35 but when SDS was used
only 11 compounds were separated because two amino compounds coeluted. |
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Keywords: | Capillary zone electrophoresis Micellar electrokinetic chromatography Aromatic sulphonate compounds |
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