| A calibration method for optical trap force by use of electrokinetic phenomena |
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| 作者姓名： | 喻有理 张镇西 张孝林 |
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| 作者单位： | Key Laboratory of Biomedical Information Engineering of Ministry of Education and Institute of Biomedical Engineering Xi'an Jiaotong University，Key Laboratory of Biomedical Information Engineering of Ministry of Education and Institute of Biomedical Engineering Xi'an Jiaotong University，School of Science Xi'an Jiaotong University，Xi'an 710049 School of Science Xi'an Jiaotong University Xi'an 710049，Xi'an 710049，Xi'an 710049 |
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| 基金项目： | This work was supported by the National Natural Science Foundation of China under Grant No. 60378018 and 60578026. |
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| 摘 要： | An experimental method for calibration of optical trap force upon cells by use of electrokinetic phenomena is demonstrated. An electronkinetic sample chamber system (ESCS) is designed instead of a common sample chamber and a costly automatism stage, thus the experimental setup is simpler and cheaper. Experiments indicate that the range of the trap force measured by this method is piconewton and sub-piconewton, which makes it fit for study on non-damage interaction between light and biological particles with optical tweezers especially. Since this method is relevant to particle electric charge, by applying an alternating electric field, the new method may overcome the problem of correcting drag force and allow us to measure simultaneously optical trap stiffness and particle electric charge.
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