药物抗氧化作用对DNA发光动力学行为的影响

本文应用CuSO₄-Phen-VC-H₂O₂-DNA化学发光体系观察药物对发光动力学行为的影响,研究了九种药物对DNA损伤的保护作用,并对其药物结构对抗氧化能力的影响进行了分析。发现药物在该系统中的抗氧化作用有三种类型,提示它们可能在体内不同的反应过程中起不同的作用。在该系统中具有抑制DNA损伤发光能力的药物,按其作用强弱依次为阿魏酸、丹参素、芦丁、茶多酚、SOD、丹参酮Ⅱ-A、丹皮酚、五味子乙素,具有延迟DNA损伤发光能力的药物,按其作用强弱依次为阿魏酸、芦丁、茶多酚、Trolox、丹参素、丹皮酚。分子结构中具有共轭大π键、羟基、羧基的药物具有较强抑制DNA损伤的能力,这可能与药物分子的氧化还原电位或自由基生成热等多种因素有关。

INFLUENCE OF ANTIOXIDANT FUNCTIONS OF DRUGS ON KINETICS OF DNA CHEMILUMINESCENCE

The influence of drugs on chemiluminescence kinetics was observed in CuSO₄ Phen V_C-H₂O₂-DNA chemiluminescent system. Nine natural drugs were studied for their protection against DNA damage and the relationship of drug structure with antioxidant activity was discussed. Three types of antioxidant functions were observed, which indicated that drugs have different functions in different reactions in vivo. In this system, the antioxidant activity of the drugs which depress DNA damage chemiluninescence decreases in the order of ferulic acid、salvianic acid A、rutin、green tea polyphenols、SOD、tans hinone Ⅱ-A、paeonol、γ-schisandrin. The antioxidant activity of the drugs which delay DNA damage chemiluminescence decreases in the order of ferulic acid、rutin、green tea polyphenols、Trolox、salvianic acid A、paeonol. The drugs containing big π conjugated system、hydroxyl group、carboxyl group have relatively great efficiency in depressing DNA damage. This is probably relative to their redox potentials.