A High Selective Chemiluminescence Reacton of Guanine and Its Analytical Application

A high selective quantitative method for the determination of guanine was established based on a well-known copper-phenanthroline complex mediated oxidation of DNA.The affecting factors for the reaction were studied in a HAc-NaAc buffer solution at 37℃. The optimized conditions for the quantitative determination of guanine are: pH5.2; Cu²⁺]=80μmol/L; ascorbic acid]=0.4μmol/L; phen]=7μmol/L and 1.1%H₂O₂.Guanine could be determined in the range of 1.0-10.0μmol/L.The recoveries of guanine in synthetic sample were within 90%-110% with up to 6 times' excess of other bases.This method is simple,fast and selective.It may be used as a routine method for the determination of guanine without the perseparation of other nucleic acid bases.