Structural determination of lysophosphatidylcholines extracted from marine sponges by fast atom bombardment tandem mass spectrometry |
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Authors: | Hong J Cho K Kim Y H Cheong C Lee K S Jung J H |
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Institution: | Hazardous Substance Research Team, Korea Basic Science Institute, Anam 5-Ga, Sungbuk-Ku, Seoul 136-701, Korea. jongki@comp.kbsi.re.kr |
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Abstract: | A series of lysophosphatidylcholines were isolated from the marine sponge Spirastrella abata by reversed-phase high performance liquid chromatography (HPLC) and analyzed by fast atom bombardment mass spectrometry (FAB-MS). Their structural elucidation was carried out with fast atom bombardment tandem mass spectrometry (FAB-MS/MS). The collision-induced dissociation (CID) of protonated and sodiated molecular ions produced diverse product ions via a series of dissociative processes. Because of the positive charge of the amine group at the end of the molecules, charge-remote fragmentation patterns of specific ions, M + H](+) or M + Na](+), were very helpful for the identification of product ions which are characteristic for choline and long hydrocarbon chains substituted at the glycerol back bone. Moreover, the CID-MS/MS spectra of sodium adducted molecular ions for lysophosphatidylcholines yielded common characteristic fragment ions for the choline moiety and characteristic ions M + Na-103](+), M + Na-85](+) and M + Na-59](+) in the higher mass region. |
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