Automated separation and quantitation of lipid fractions by high-performance liquid chromatography and mass detection |
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| Authors: | P R Redden Y S Huang |
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| Institution: | Efamol Research Institute, Kentville, Nova Scotia, Canada. |
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| Abstract: | This report describes an improved separation and quantitation of lipid fractions in a total lipid extract by high-performance liquid chromatography using a modified solvent and gradient system delivered by dual pumps and incorporating a mass detector and autosampler. The detector responses for various lipid fractions (cholesteryl esters, triacylglycerols, free cholesterol, and seven major phospholipid classes) were fitted to a quadratic equation, y = ax2 + bx + c, and quantified after detector calibration by a computer. This new system has the advantage of automation and reproducible separation. The present method was applied to rat liver analysis. |
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