Anion-exchange and size-exclusion chromatography of proteins in mouse ascites fluid: Changes in the protein pattern during tumour growthIn vivo |
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Authors: | A Werner W Siems U Kuckelkorn T Grune C Schreiter |
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Institution: | (1) Institute of Biochemistry, Medical Faculty (Charite), Humboldt-University of Berlin, Hessische Str. 3/4, 1040 Berlin, GDR |
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Abstract: | Summary During the transition of Ehrlich mouse ascites tumour cells from the proliferating to the resting state of growth a large loss of purine and pyrimidine compounds occurs. This decrease is accompanied by a change in the amount of protein in the supernate of ascites fluid, which is known from the study of the ATP-consumption during protein synthesis. The ascites fluid was investigated by anion-exchange and size-exclusion chromatography (SEC). SDS-PAGE (sodium, dodecyl sulfate-polyacrylamide gel electrophoresis) data were compared with SEC data. The total amount of protein increased by 50% between day 5 and 12 of growth. At least 5 new peaks are observed in the chromatograms of an ion-exchange separation of Ehrlich ascites fluid at day 12 postinoculation. The amounts of transferrin, albumin and IgG (immunoglobulin G) were increased to 132, 134, 157%, respectively duringin vivo growth. |
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Keywords: | Column liquid chromatography Anion exchange Size exclusion Ehrlich ascites fluid proteins |
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