| PAOAA修饰金电极固定过氧化氢酶的电化学研究 |
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| 引用本文： | 张志军,李曦,肖亮泓,邹传亮,张超灿.PAOAA修饰金电极固定过氧化氢酶的电化学研究[J].中国科学:化学,2011(6):1001-1009. |
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| 作者姓名： | 张志军 李曦 肖亮泓 邹传亮 张超灿 |
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| 作者单位： | 武汉理工大学理学院;武汉理工大学材料科学与工程学院; |
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| 摘 要： | 设计了一种基于金属离子螯合法固定蛋白质的新方法.首先电化学聚合苯胺(ANI)/邻氨基苯甲酸(OAA)得到在中性溶液中具有导电性的聚(苯胺-邻氨基苯甲酸)(PAOAA)共聚物膜,并对膜进行了SEM、EDS表征.随后膜负载Cu2+,Cu2+作为螯合离子通过配位作用固定过氧化氢酶(Cat).用EDS测量了铜的负载量为0.49%.脉冲伏安法(DPV)研究了Cat在金电极表面的固定过程,显示随着ANI/OAA在电极表面聚合成膜、Cat在修饰电极表面固定,电极在10mmol/LK3Fe(CN)6/K4Fe(CN)6(Fe(CN)63-/4-)溶液中的扫描峰电流由裸电极的121μA依次降到92.6、71.8μA,表明电极表面修饰的PAOAA膜和固定的Cat阻碍了电荷的传递.电化学阻抗法(EIS)对固定过程中电极表面阻抗变化的研究也得到同样结论.用线性扫描伏安法(LSV)研究了固定到电极表面后Cat对H2O2的催化还原活性.最后以计时电流法(CA)作为检测手段,Cat修饰电极作为探针实现了对H2O2的定量检测,同时该电极显示出良好的重建性.
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| 关 键 词： | 聚(苯胺-邻氨基苯甲酸) 电化学聚合 蛋白质固定 金属离子螯合 过氧化氢酶 |
Electrochemical study of Catalase immobilized on PAOOA-modified gold electrode |
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| ZHANG ZhiJun ,LI Xi ,XIAO LiangHong ,ZOU ChuanLiang &ZHANG ChaoCan School of Science,Wuhan University of Technology,Wuhan ,China School of Materials Science , Engineering,China.Electrochemical study of Catalase immobilized on PAOOA-modified gold electrode[J].Scientia Sinica Chimica,2011(6):1001-1009. |
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| Authors: | ZHANG ZhiJun LI Xi XIAO LiangHong ZOU ChuanLiang &ZHANG ChaoCan School of Science Wuhan University of Technology Wuhan China School of Materials Science Engineering China |
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| Institution: | ZHANG ZhiJun 1,LI Xi 1,XIAO LiangHong 1,ZOU ChuanLiang 1 &ZHANG ChaoCan 2 1 School of Science,Wuhan University of Technology,Wuhan 430070,China 2 School of Materials Science and Engineering,China |
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| Abstract: | A new method for protein immobilization based on metal-ion chelation was reported in this paper.First,conductivity film poly(aniline-co-2-aminobenzoic acid)(PAOAA),which is able to conductive in neutral solution,was prepared by electrochemical deposition.The film was characterized by scanning electron microscopy(SEM)and energy-dispersive X-ray(EDS).Then Cu2+ions were adsorbed on the film as the chelating ion to immobilize catalase(Cat).The molar amount of Cu2+ions in the polymer was 0.49%measured by EDS.Dif... |
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| Keywords: | poly(aniline-co-2-aminobenzoic acid) electrochemical polymerization protein immobilization metal ion chelation Catalase |
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