In situ monitoring of antibiotic susceptibility of bacterial biofilms in a microfluidic device |
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Authors: | Kim Keun Pil Kim Yun-Gon Choi Chang-Hyung Kim Hye-Eun Lee Sang-Ho Chang Woo-Suk Lee Chang-Soo |
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Institution: | Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA. |
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Abstract: | Antibiotic resistance of biofilms is a growing public health concern due to overuse and improper use of antibiotics. Thus, determining an effective minimal concentration of antibiotics to eradicate bacterial biofilms is crucial. Here we present a simple, novel one-pot assay for the analysis of antibiotic susceptibility of bacterial biofilms using a microfluidics system where continuous concentration gradients of antibiotics are generated. The results of minimal biofilm eradication concentration (MBEC) clearly confirm that the concentration required to eradicate biofilm-grown Pseudomonas aeruginosa is higher than the minimal inhibitory concentration (MIC) that has been widely used to determine the lowest concentration of antibiotics against planktonically grown bacteria. |
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