直接进样电喷雾串联质谱法测定草鱼肌肉组织中磷脂

建立了直接进样电喷雾串联质谱测定草鱼肌肉组织中磷脂的方法.以Bligh Dyer法提取总脂质,采用流动注射泵直接进样的方式将样品导人电喷雾离子源,利用串联三重四级杆质谱的母离子扫描和中性丢失扫描功能,通过扫描磷脂的特征性子离子或中性质量丢失实现对磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰肌醇、磷脂酰丝氨酸、磷脂酰甘油和磷脂酸六类磷脂的源内分离和鉴定.结果显示,在-定的浓度范围内,磷脂的浓度与磷脂直接进样电喷雾电离后形成准分子离子的响应值呈现良好的线性关系,回收率(67.1％～96.6％)和精密度可以满足生物样品分析的要求.采用本方法测定了草鱼肌肉组织中磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰肌醇和磷脂酰丝氨酸4类磷脂的分子种及含量.本方法前处理简单,定性和定量分析快速准确,可以应用于其它生物样本脂质组学中磷脂的分析.

Determination of Phospholipids from Ctenopharyngodon Idellus Muscle by Direct-injection Electrospray Ionization Tandem Mass Spectrometry

A method was developed for the determination of phospholipids from Ctenopharyngodon idellus muscle by direct-injection electrospray ionization tandem mass spectrometry(ESI-MS/MS).The samples were extracted with modified Bligh Dyer method and the crude extracts were taken directly into the electrospray ionization source by syringe pump.Under the precursor ion scan and neutral loss scan,intrasource separation and identification of six classes of phospholipids including phosphatidyl choline,phosphatidyl ethanolamine,phosphatidyl inositol,phosphatidyl serine,phosphatidyl glycerol and phosphatidic acid were achieved.The intensity of quasi-molecular ion of the phospholipids and concentration showed good linear relation in a certain range.The recoveries(67.1%-96.6%) and precision meet the requirements for analyzing biological samples.This method has been applied to analyze the molecular species and content of four class phospholipids from Ctenopharyngodon idellus muscle.The method is simple,fast accurate,and reproducibility with excellent stability,and suitable to analyze phospholipids from various biological samples for lipidomics research.