化学发光免疫分析方法与应用进展

化学发光免疫分析方法具有选择性好、灵敏度高、分析速度快、设备简单等特点,在环境、临床、食品、药物检测等领域得到了广泛应用。在实际应用中,常常需要对大量复杂样品或低丰度分析物样品进行测定。而经典商品化的免疫分析方法所需时间长、样品消耗多、成本高,不能满足上述要求。因而建立快速、高通量、高灵敏和低成本的免疫检测方法已成为化学发光免疫分析方法的研究热点和发展趋势。本文简要总结近5年来化学发光免疫分析方法的研究进展及其应用,并展望其发展方向。

Chemiluminescent Immunoassay and Its Application

Chemiluminescent immunoassay(CLIA) has been applied in different fields including environmental,clinical diagnosis,food safety,and pharmaceutical analysis as a promising approach for selective,sensitive,rapid and simple analysis.It is often necessary to detect a large number of complicated or low abundance samples.However,traditional method needs great consumptions of time,reagent and labor,which limit their clinical applications.As a result,building rapid,high throughput,sensitive and low cost detection methods has become the development trend for CLIA.In this review,we summarize the recent advances in CLIA and its application,and point out the future development of CLIA.