离子色谱-脉冲安培电化学检测引流组织液中的妥布霉素

建立了一种离子色谱(IC)分离,脉冲安培电化学法(PAD)直接检测人体引流组织液中妥布霉素的分析方法.采用高交换容量阴离子交换柱和低浓度KOH在线淋洗分离,不需要采用衍生化和离子对-柱后加碱等实现分离,并可在短时间内完成妥布霉素的测定.采用本方法测定的妥布霉素线性范围为0.05～10 mg/L,线性相关系数为0.9997,保留时间、峰面积和峰高的相对标准偏差分别为0.14％,0.38％和0.81％,检出限为7.11 μg/L.本方法成功应用于骨髓炎患者引流组织液中妥布霉素的测定,样品实际加标回收率为100.8％.

Analysis of Tobramycin in Human Drainage Tissue Fluid by Ion Chromatography with Pulsed Integrated Amperometric Detection

A new method was developed for the separation and determination of tobramycin in human drainage tissue fluid by ion chromatography(IC) with pulsed integrated amperometric detection(PAD).Tobramycin was separated on an anion-exchange column of high capacity using a weak potassium hydroxide solution as eluent.In comparison with the previous methods,derivatization and ion-pairing with post-column addition of NaOH were not required in this method.Under the optimum conditions,the linear range of the calibration curve for tobramycin was 0.05-10 mg/L(r=0.9997),whereas detection limit was 7.11 μg/L(S/N=3).The spiked recovery was 100.8% and the relative standard deviations for retention time,peak area and peak height were 0.14%,0.38% and 0.81% respectively.The method was successfully applied to determine tobramycin in human drainage tissue fluid of patient who suffered from traumatic osteomyelitis.