心脏胶原蛋白中交联氨基酸的质谱分析

心脏衰竭可能与心脏胶原蛋白交联异常有关,因为交联过程对心脏胶原蛋白的机械强度起决定作用.研究表明,某些交联氨基酸的含量可以用作对应胶原蛋白交联异常的分子标记,但心脏胶原蛋白交联异常的分子标记尚不清楚.本研究建立了串联质谱的分析方法可以高选择性的检测水解胶原蛋白质中两种重要的交联氨基酸-吡啶诺林(PYD)和去氧吡啶诺林(DPD).在小鼠心室胶原蛋白酸性水解产物中检测到DPD,未检测到PYD,说明PYD与心脏胶原蛋白的交联过程无关,而DPD在心脏胶原蛋白交联中起更重要的作用,有可能成为与心脏衰竭相关的分子标记.本研究提供了一种基于质谱的研究心脏胶原蛋白交联产物的方法,也可用于其它胶原蛋白的分析.

Mass Spectrometric Analysis of Cross-linked Amino Acids in Collagen

Cross-linking plays a critical role in determining the mechanical strength of heart collagen,and its malfunction is probably related to the occurrence of heart failure.Studies show that the content of some cross-linked amino acids can be used as biomarkers for the abnormal cross-linking of their corresponding collagens.However,it is still unclear about the biomarkers for the unusual cross-linking in heart collagen.Hence we present a tandem mass spectrometry method that enables highly selective identification of two important crosslink,amino acids-pyridinoline(PYD) and deoxypyridi-noline(DPD) in hydrolyzed collagen.DPD is detected in acid hydrolyzed mouse ventricle collagen,while PYD not,indicating that PYD is not related to the cross-linking processes in heart collagen,but DPD plays a more significant role,so DPD might become a biomarker for heart failure.Overall,this study provides a mass spectrometry based method to investigate the cross-linking products in mouse heart collagen,which can be also applied to other collagens