Design of liposomes containing photopolymerizable phospholipids for triggered release of contents |
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| Authors: | Amichai Yavlovich Alok Singh Sergey Tarasov Jacek Capala Robert Blumenthal Anu Puri |
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| Affiliation: | (1) Membrane Structure and Function Section, Center for Cancer Research Nanobiology Program, NCI-Frederick, NIH, Frederick, MD 21702, USA;(2) Naval Research Laboratory, Center for Bio/Molecular Science and Engineering, Washington DC, USA;(3) Structural Biophysics Laboratory, Center for Cancer Research, NCI-Frederick, NIH, Frederick, MD 21702, USA;(4) Center for Cancer Research, NCI-Bethesda, NIH, Bethesda, MD 20892, USA; |
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| Abstract: | We describe a novel class of light-triggerable liposomes prepared from a photo-polymerizable phospholipid DC8,9PC (1,2-bis (tricosa-10,12-diynoyl)-sn-glycero-3-phosphocholine) and DPPC (1,2-Dipalmitoyl-sn-Glycero-3-Phosphocholine). Exposure to UV (254 nm) radiation for 0–45 min at 25 °C resulted in photo-polymerization of DC8,9PC in these liposomes and the release of an encapsulated fluorescent dye (calcein). Kinetics and extents of calcein release correlated with mol% of DC8,9PC in the liposomes. Photopolymerization and calcein release occurred only from DPPC/DC8,9PC but not from Egg PC/DC8,9PC liposomes. Our data indicate that phase separation and packing of polymerizable lipids in the liposome bilayer are major determinants of photo-activation and triggered contents release. |
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