Determination of adenosine deaminase and purine nucleoside phosphorylase activities using high-performance liquid chromatographic and radiochromatographic methods |
| |
| Authors: | J Bohácek B Hosek O Babusíková A Hrivnáková |
| |
| Institution: | Institute of Biophysics, Czechoslovak Academy of Sciences, Brno. |
| |
| Abstract: | Two methods for the determination of adenosine deaminase and purine nucleoside phosphorylase activities were compared. The high-performance liquid chromatographic (HPLC) technique used separation on a reversed-phase silica column and exhibited adequate sensitivity and a markedly higher rate of analysis compared with that of the paper radiochromatographic method. Correlation analysis of the results obtained by the two methods on a set of lymphoid cells from 25 patients with lympho-proliferative disorders confirmed the utility of the HPLC technique in clinical investigations. |
| |
| Keywords: | |
|
