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| 5种体色瓯江彩鲤线粒体COII基因的序列差异和遗传标记研究 | |
| 引用本文： | 吕耀平,胡则辉,肖建中,王成辉,胡金强,劳沈颖,叶丽平,项松平,刘青娥. 5种体色瓯江彩鲤线粒体COII基因的序列差异和遗传标记研究[J]. 浙江大学学报(理学版), 2009, 36(3): 323-327. DOI: 10.3785/j.issn.1008-9497.2009.03.018 |
| 作者姓名： | 吕耀平 胡则辉 肖建中 王成辉 胡金强 劳沈颖 叶丽平 项松平 刘青娥 |
| 作者单位： | 1. 丽水学院,化学与生命科学学院,浙江,丽水,323000;浙江大学,动物科学学院,浙江,杭州,310029 2. 浙江省海洋水产研究所,浙江,舟山,316100 3. 丽水学院,化学与生命科学学院,浙江,丽水,323000 4. 上海水产大学农业部水产种质资源与养殖生态重点开放实验室,上海,200090 5. 都灵大学兽医学院,意大利,皮埃蒙特,10095 6. 浙江龙泉省级瓯江彩鲤良种场,浙江,龙泉,323700 7. 丽水学院化学与生命科学学院,浙江,丽水,323000 |
| 基金项目： | 浙江省自然科学基金，浙江省科技厅项目 |
| 摘 要： | 利用PCR方法扩增获得瓯江彩鲤（Cyprinus carpio var．color）线粒体DNA的COⅡ基因，测定该基因片段序列．分析了瓯江彩鲤“全红”、“粉玉”、“粉花”、“麻花”和“大花”共20只个体的序列核苷酸位点差异和遗传多态性．结果表明，“大花”和“全红”遗传多样性最为丰富，“粉花”的遗传多样性相对贫乏．在长度为604bp的基因片段中，共检测到9个多态性核苷酸位点（占1．49%），20只个体具有4种基因型，5种体色各自的平均核苷酸位点差异分别为0．38％、0．2％、0、0．13％和0．47％．UPGMA分子系统聚类树显示，“粉花”、“麻花”和“粉玉”的遗传关系接近；“全红”相对独立成一支，与其他4种体色瓯江彩鲤的遗传关系较远．COⅡ基因可以作为区分三分支群体的遗传标记． |
| 关 键 词： | 瓯江彩鲤 COⅡ基因 序列多态性 遗传标记 |
Intraspecific DNA sequence polymorphism and genetic markers in the mitochondrial COII gene from five color patterns of Oujiang color carp |
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| LV Yao-ping,HU Ze-hui,XIAO Jian-zhong,WANG Cheng-hui,HU Jin-qiang,LAO Shen-ying,YE Li-ping,XIANG Song-ping,LIU Qin-ge. Intraspecific DNA sequence polymorphism and genetic markers in the mitochondrial COII gene from five color patterns of Oujiang color carp[J]. Journal of Zhejiang University(Sciences Edition), 2009, 36(3): 323-327. DOI: 10.3785/j.issn.1008-9497.2009.03.018 | |
| Authors: | LV Yao-ping HU Ze-hui XIAO Jian-zhong WANG Cheng-hui HU Jin-qiang LAO Shen-ying YE Li-ping XIANG Song-ping LIU Qin-ge |
| Affiliation: | LV Yao-ping , HU Ze-hui , XIAO Jian-zhong , WANG Cheng-hui , HU Jin-qiang , LAO Shen-ying , YE Li- ping , XIANG Song-ping , LIU Qing-e (1. College of Chemistry and Life Sciences, Lishui University, Lishui 323000, Zhejiang Province, China; 2. College of Animal Sciences, Zhejiang University, Hangzhou 310029, China ; 3. Marine Fisheries Research Institute of Zhej iang Province, Zhoushan 316100, Zhejiang Province, China ; 4. Key Laboratory of Aquatic Genetic Resources and Aquacultural Ecosystem Certificated by the Ministry of Agriculture, Shanghai Fisheries University, Shanghai 200090, China; 5. Dipartimento di Produzioni Animali, Epidemiologia ed Ecologia, Facolta di Medicina Veterinaria, Universita di rorino, Grugliasco (TO) 10095, Italy; 6. Provincial Farm of Oujiang Color Common Carp, Longquan 323700, Zhejiang Province, China) |
| Abstract: | The mitochondrial COII gene was PCR proliferated and sequenced from 20 samples obtained from five color patterns of Oujiang color carp,designated as whole red,red with scattered black spots,whole white and white with scattered black spots.A 604 bp long partial gene segment was acquired and used to study the genetic diversity among the five color patterns.Results indicated that the COII gene locus was relatively more polymorphic in the red color with large black patches(RP) and whole red(WR),while the polymo... |
| Keywords: | Cyprinus carpio var.color COII gene sequence polymorphism genetic marker |
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