Electrochemical Reactions During Isoelectric Focusing and Their Role in Establishment of the pH Gradient |
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| Authors: | Cyrill L. Naydenov Evgeni P. Kirazov Valentin S. Lozanov Ludmil P. Kirazov Vanio I. Mitev |
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| Affiliation: | 1. Department of Chemistry and Biochemistry, Medical University Sofia, 2 Zdrave Str., 1431, Sofia, Bulgaria
2. Institute of Experimental Morphology and Anthropology with Museum, Bulgarian Academy of Sciences, Acad. G. Bonchev Str. Bl. 25, 1113, Sofia, Bulgaria
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| Abstract: | The process occurring as the background to IEF is electrolysis of water. During IEF the yield of water ions decreases, following a non-linear relationship similar to that of the current. Different extents of acidification and basification of the electrode solutions, causing a drift of the pH gradient, were observed. We derived a relationship underlying this process which enables calculation of the electrode current. By using a novel approach we showed that the sum of the pH of distilled water electrode solutions at the end of the IEF process tends to the ionic product of water. |
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