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Development of a Sensitive LC Assay with Fluorescence Detection for the Determination of Zearalenone in Rat Serum |
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| Authors: | Beom Soo Shin Seok Hyun Hong Hyoung Jun Kim Hae-Seong Yoon Do Jung Kim Sang Wook Hwang Jong Bong Lee Sun Dong Yoo |
| Institution: | 1. College of Pharmacy, Catholic University of Daegu, Gyeongsan-si, Gyeongbuk, South Korea 2. College of Pharmacy, Sungkyunkwan University, Suwon, Gyeonggi-do, South Korea 3. Human Exposure Assessment Division, National Institute of Toxicological Research, Eunpyung-ku, Seoul, South Korea |
| Abstract: | A simple and sensitive liquid chromatographic assay with fluorescence detection assay was developed for the determination of zearalenone levels in rat serum. The assay utilized a single liquid–liquid extraction with t-butyl methyl ether and isocratic elution using a mobile phase consisting of acetonitrile and 0.1% triethylamine in distilled water (pH = 6) (50:50, v/v). Linearity was observed over a concentration range from 10 to 1,000 ng mL?1 (r = 0.9995), with the limit of quantification at 10 ng mL?1 with 100 μL of rat serum. The validated assay was applied to a pharmacokinetic study in rats. |
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