Determination of Tangeretin in Rat Plasma by LC-Electrospray-Ion Trap MS |
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Authors: | Da-Yong Zhou De-Liang Chen Xian-Dong Yang Qing Xu Xing-Ya Xue Fei-Fang Zhang Xin-Miao Liang |
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Affiliation: | 1. College of Bio and Food Technology, Dalian Polytechnic University, 116034, Dalian, People’s Republic of China 2. Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 116023, Dalian, People’s Republic of China 3. Division of Cardiology, Department of Internal Medicine, Wuhan University ZhongNan Hospital, 430071, Wuhan, People’s Republic of China 4. Dalian Medical University, 116027, Dalian, People’s Republic of China
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Abstract: | A selective, sensitive, and accurate method has been developed and validated for the quantification of tangeretin in rat plasma. The application of LC-electrospray-ion trap mass spectrometry in full scan and multiple reactions monitoring modes were investigated. Following solid phase extraction using a hydrophilic–lipophilic balance cartridge, the analytes were separated on a C18 column using an isocratic mobile phase composed of acetonitrile/water (50:50, v/v) containing 0.3% formic acid. In full scan mode, the LOQ was 2 ng mL?1. The standard calibration curve was linear (R 2 = 0.9999) over the concentration range 2–200 ng mL?1. The precision over the concentration range was within 15% (RSD) and the accuracy was ranged from 86 to 115%. In multiple reaction monitoring mode, the LOQ was 1 ng mL?1 and the standard calibration curve was linear (R 2 = 0.9976) over the concentration range 1–100 ng mL?1 with a precision of 12% and accuracy rangeing from 91 to 113%. |
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