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Enzymatic mechanisms and electron transfer mediation in chronoamperometric biosensors
Authors:Joseph Jordan  M. K. Ciolkosz
Affiliation:1. Department of Chemistry, The Pennsylvania State University, 152 Davey Laboratory, 16802, University Park, PA
Abstract:Electron transfer mediation to an anode, whose potential is judiciously controlled, provides the conceptual basis for the development of novel chronoamperometric biosensors. The mediators are appropriately selected redox couples (Ox/Red) which are amenable to recycling in the following type of enzyme E catalyzed reaction sequence

$$begin{gathered}  {text{Substrate  +  Ox}}{}^{text{E}} to {text{ Product  +  Red }} hfill   {text{Red }} to {text{ Ox  +  }}ne^ -  {text{ }} hfill  end{gathered}$$
Keywords:Alcohol oxidase  biosensors  chronoamperometry  electron transfer mediation  enzymatic redox mechanisms  glusose oxidase  pseudo-oxidase, substrate/co-substrate selectivity
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