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Electron ionization-tandem mass spectrometry of glycosphingolipids. I: The identiftcation of compound-specific sequence ions in the collision-induced dissociation spectra of the immonium ions of two isomeric hexaglycosylceramides |
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| Authors: | J M Curtis P J Derrick J Holgersson B E Samuelsson M E Breimer |
| Institution: | 1. Warwick Institute for Mass Spectrometry, University of Warwick, CV4 7AL, Coventry, UK 2. Department of Chemistry, University of Warwick, CV4 7AL, Coventry, UK 3. Department of Medical Biochemistry, University of G?teborg, G?teborg, Sweden 4. Department of Surgery, Sahlgrerr’s Hospital, University of G?teborg, G?teborg, Sweden |
| Abstract: | A permethylated-reduced hexaglycosylceramide in a complex glycolipid mixture isolated from a unique human tissue has been identified by using tandem mass spectrometry (MS/MS). The mass spectrum of this glycolipid mixture, obtained by using in-beam electron ionization, is very complex, and fragment ions derived from the hexaglycosylceramide cannot be distinguished from other ions. Tandem mass spectrometry using a four-sector mass spectrometer gave the mass spectrum of the immonium ion of the permethylated-reduced hexaglycosykeramide (m / z 1645.8), which is characteristic of its structure. Comparison of this MS/MS spectrum with those of two similarly derivatized blood group hexaglycosylceramide isomers permitted identification of the unknown glycolipid structure. |
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