pH-based cation exchange chromatography in the capture and elution of monoclonal antibodies |
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Authors: | Ng Paul K Snyder Mark A |
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Affiliation: | Process Applications R&D, Process Chromatography Division, Bio-Rad Laboratories, Hercules, CA 94547, USA. paul_ng@bio-rad.com |
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Abstract: | Cation exchange chromatography separates adsorbed proteins by controlling the salt concentration or the mobile phase pH. This study examines the pH‐based method for binding and elution of monoclonal antibodies (MAbs). Five different clones with isoelectric points from 6 to 9 were evaluated. We performed our studies using a new chromatography resin (Nuvia? S), which has high binding capacity. A three‐column process incorporating Nuvia S as a capture step was also demonstrated for the purification of MAb from tissue culture fluid. Chromatography performance of Nuvia S was demonstrated in a 50‐cycle study. |
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Keywords: | Antibody Cation exchange Elution Nuvia S pH |
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