| 一个肺癌相关新基因ASPH6功能的初步研究 |
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| 引用本文： | 付晓星,王静,李娜,陈玥,何大澄,孙锁柱,肖雪媛.一个肺癌相关新基因ASPH6功能的初步研究[J].北京师范大学学报(自然科学版),2010,46(4). |
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| 作者姓名： | 付晓星 王静 李娜 陈玥 何大澄 孙锁柱 肖雪媛 |
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| 作者单位： | 北京师范大学细胞增殖与调控教育部重点实验室,高等学校蛋白质组学研究院,100875,北京;第二炮兵总医院病理科,100088,北京 |
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| 基金项目： | 2009年细胞增殖与调控教育部重点实验室开放课题资助项目 |
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| 摘 要： | 为了探讨ASPH6在肺癌发生和发展中的生物学作用,构建了ASPH6真核表达载体,分别转染了2株肺腺癌细胞系LTEP-a-2和NCI-H1299,经G418筛选后获得了稳定的克隆细胞株.体外实验结果显示,表达ASPH6的肺癌细胞与对照空载细胞相比,细胞的迁移和浸润能力明显下降,但细胞的生长无明显改变.动物体内实验性转移结果显示,高表达ASPH6可明显抑制肺癌细胞的转移能力.进一步通过蛋白质组学方法,筛选并鉴定出14个ASPH表达调控蛋白.因此推测,ASPH6很可能是一个在肺癌发生和发展中起负调控作用的基因.
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| 关 键 词： | ASPH6 肺腺癌 转移 |
FUNCTION OF GENE ASPH6, A NOVEL ASPH VARIANT |
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| FU Xiaoxing,WANG Jing,LI Na,CHEN Yue,HE Dacheng,SUN Suozhu,XIAO Xueyuan.FUNCTION OF GENE ASPH6, A NOVEL ASPH VARIANT[J].Journal of Beijing Normal University(Natural Science),2010,46(4). |
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| Authors: | FU Xiaoxing WANG Jing LI Na CHEN Yue HE Dacheng SUN Suozhu XIAO Xueyuan |
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| Institution: | FU Xiaoxing1) WANG Jing 1) LI Na1) CHEN Yue1) HE Dacheng1) SUN Suozhu2) XIAO Xueyuan1)(1)Key Laboratory of Cell Proliferation and Regulation Biology Ministry of Education,Universities Confederated Institute of Proteomics,Beijing Normal University,100875,Beijing,China,2)Department of Pathology,General Hospital of the Second Artillery,PLA,100088,China) |
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| Abstract: | To elucidate ASPH6 function and corerelation between ASPH6 and lung cancer,a full length ASPH6 expression vector was constructed and stably transfected into lung adenocarcinoma cell lines LTEP-a-2 and NCI-H1299.After selection with G418,clones stably over-expressing ASPH6 were obtained.In migration assay and scratch wounding,it was found that lung cancer cells over-expressing ASPH6 demonstrated reduced motility and invasive capacity,but no significant changes in cell grow rate were found in comparison with ... |
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| Keywords: | ASPH6 |
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