A study of magnetic field effects on fibroblast cultures Part 1. The evaluation of the effects of static and extremely low frequency (ELF) magnetic fields on vital functions of fibroblasts |
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| Institution: | 1. São Paulo''s Agency for Agribusiness Technology/São Paulo''s Agency Agriculture and Food Suply, APTA/SAA, Ribeirão Preto, SP, Brazil;2. Graduate Program in Animal Science—PPGCA, School of Life Sciences, Pontifical Catholic University of Parana—PUCPR, Curitiba, PR, Brazil;3. College of Veterinary Medicine (FMVA), São Paulo State University (UNESP), Araçatuba, SP, Brazil;4. College of Veterinary Medicine (FMVA), São Paulo State University (UNESP), Jaboticabal, SP, Brazil;5. Professor de College of Veterinary Medicine, University from São Paulos''s West (UNOESTE), São Paulo, SP, Brazil;1. Dept. of Chemical and Environmental Process Engineering, Budapest University of Technology and Economics, Budafoki út 8. F II. ,Budapest H-1111, Hungary;2. Department of Fine Chemicals and Environmental Technologies, University of Miskolc, H-3515, Miskolc, Egyetemváros, Hungary;1. Laboratory of Development in Mechanics and Materials, LDMM, Faculty of Sciences and Technology, University of Djelfa, Djelfa, Algeria;2. Department of Mechanical Engineering, Celal Bayar University, 45140 Manisa, Turkey |
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| Abstract: | Cultured fibroblasts isolated from murine livers by tissue trypsinization were exposed to a static magnetic field (0.49 T) and an extremely low frequency (ELF) magnetic field (50 Hz, 0.020 T). The cultures were exposed to magnetic fields on four consecutive days for exposure times of 2, 4, 8, 16, 32 and 64 min. After exposure the following parameters of the fibroblast cultures were determined: the dynamics of culture growth, the protein content and 14C-thymidine incorporation. The cytometric parameters of the fibroblasts were also assessed. The ELF magnetic field compromised vital functions of the fibroblasts (inhibition of culture growth, decreased cellular protein, lowered cytometric parameters) and caused a slowdown in the rate of 14C-thymidine incorporation which indicates altered DNA synthesis. Ongoing experiments with a static magnetic field have shown no effect on the vital functions of fibroblasts. |
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