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Induced Color Change of Conjugated Polymeric Vesicles by Interfacial Catalysis of Phospholipase A2 |
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| Authors: | Sheldon Yoshio Okada Raz Jelinek Deborah Charych |
| Abstract: | A blue to red color change is induced on addition of phospholipase A2 to modified PDA vesicles 1 (PDA=polydiacetylene). This bathochromic transition results from chemical modification of the vesicles by hydrolysis of the enzyme substrate embedded in the PDA matrix. Addition of a known phospholipase inhibitor or removal of Ca2+ ions suppresses the color change, which suggests the potential for applications in high‐throughput screening assays. |
| Keywords: | Biosensors Conjugation Enzyme catalysis Enzyme inhibitors Vesicles |