Gas chromatographic-mass spectrometric assay for 2-methoxyethanol and 2-methoxyacetic acid in human plasma and its application to pharmacokinetic studies |
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| Authors: | Tung-Sheng Shih Ryh-Nan Pan Jui-Shu Chou Cheng-Yao Chen Yoa-Pu Hu |
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| Institution: | (1) Institute of Occupational Safety and Health, Council of Labor Affairs, 4th Fl, No. 132 Min-Sheng E. Rd., Section 3, Taipei, Taiwan, Republic of China;(2) Department of Fire Protection, Taiwan Police College, No. 153 Shing-Lung Rd., Section 3, Taipei, Taiwan, Republic of China;(3) Pharmaceutical Research Institute, National Defense Medical Center, P.O. Box 90048-512, Taipei, Taiwan 100, Republic of China |
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| Abstract: | Summary A new assay has been developed for simultaneous measurement of 2-methoxyethanol (ME) and its major toxic metabolite, 2-methoxyacetic
acid (MAA) in human blood by gas chromatography-mass spectrometry. Over the concentration range 0.3 to 200 μgml−1, ME and MAA could be determined with a pooled coefficient of variation (eleven concentrations, six replicate samples) of
6.5% and 8.7%, respectively. The limits of quantification of ME and MAA were 0.19 and 0.05μg mL−1, respectively. The respective mean (SD) recoveries of ME and MAA were 101.3 (2.6)% and 98.2 (4.9)% (n=3). A study of the effects of human contact with ME liquid showed that this new method is a specific, sensitive, simple,
and reliable method both for the pharmacokinetic studies and for the biological monitoring of occupational exposure to ME. |
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| Keywords: | Gas chromatography-mass spectrometry 2-methoxyethanol 2-Methoxyacetic acid Blood samples Occupational hygiene |
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