Enhancing detectability in CE by combining an isotachophoretic preconcentration with capillary zone electrophoresis in a single capillary

Summary This paper describes a strategy where a major part of a single capillary is filled with sample which when is isotachophoretically (ITP) preconcentrated while a hydrodynamic backpressure is applied to keep the analytes in the capillary. In the subsequent analysis of the test compounds, amitriptyline and metoprolol, capillary zone electrophoresis is used. The concentration limit of detection is lowered at least 170 times. Preliminary results where an ITP-preconcentration is combined with micellar electrokinetic chromatography with a neutral micellar agent is also presented.