Bacitracin production by a new strain ofBacillus subtilis |
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Authors: | E. C. Azevedo E. M. Rios K. Fukushima G. M. Campos-Takaki |
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Affiliation: | (1) Mestrado de Ciencias Farmacêuticas, Universidade Federal de Pernambuco, 50739 Recife-PE, Brazil;(2) Depto. de Antibióticos, Universidade Federal de Pernambuco, 50739 Recife-PE, Brazil;(3) Laboratório de Imunopatologia Prof. Keizo Asami-LIKA, Universidade Federal de Pernambuco, 50739 Recife-PE, Brazil |
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Abstract: | A new strain ofBacillus subtilis C 126 was isolated from sugar cane fermentation and produced an antibiotic that inhibited the growth ofMicrococcus flavus. The production of the antibiotic in culture medium followed to extraction withn-butanol, thin layer chromatography, and microbiological tests indicated that a polypeptide antibiotic was produced. The fraction obtained by Sephadex G-25 column and analyzed by HPLC indicated that bacitracin complex was produced. |
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Keywords: | Bacillus subtilis bacitracin production Micrococcus flavus |
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