| THERMAL ACTIVATION OF IMMOBILIZED PAPAIN |
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| 作者姓名： | 卓仁禧 贺枫 刘立建 徐勉懿 |
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| 作者单位： | Department of Chemistry，Wuhan University，Wuhan 430072，China，Department of Chemistry，Wuhan University，Wuhan 430072，China，Department of Chemistry，Wuhan University，Wuhan 430072，China，Department of Chemistry，Wuhan University，Wuhan 430072，China |
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| 基金项目： | This research was supported by the Foundation of State Education Committee of China. |
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| 摘 要： | Papain (Papainase, EC 3.4.22.2) was immobilized on porous silica beadsby cross linking with glutaraldehyde. The thermal activation of this immobilized papainin aqueous system was found at a temperature range from 50 to 90℃. The higher thetemperature, the more active the immobilized papain will possess. At the same time,the durability of the immobilized papain on heating was greatly improved. The effect ofadditives and salts on the activity of the immobilized papain were also studied. The resultsshowed that the additives and some of the salts studied could markedly enhance the activityof the immobilized papain at elevated temperature.
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| 收稿时间： | 1997-03-25 |
THERMAL ACTIVATION OF IMMOBILIZED PAPAIN* |
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| ZHUO Renxi,HE Feng,LIU Lijian,XU Mianyi.THERMAL ACTIVATION OF IMMOBILIZED PAPAIN[J].Chinese Journal of Polymer Science,1998,0(2):142-146. |
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| Authors: | ZHUO Renxi HE Feng LIU Lijian XU Mianyi |
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| Institution: | Department of Chemistry; Wuhan University; Wuhan 430072; China |
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| Abstract: | Papain (Papainase, EC 3.4.22.2) was immobilized on porous silica beadsby cross linking with glutaraldehyde. The thermal activation of this immobilized papainin aqueous system was found at a temperature range from 50 to 90℃. The higher thetemperature, the more active the immobilized papain will possess. At the same time,the durability of the immobilized papain on heating was greatly improved. The effect ofadditives and salts on the activity of the immobilized papain were also studied. The results showed that the additives and some of the salts studied could markedly enhance the activity of the immobilized papain at elevated temperature. |
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| Keywords: | Papain Immobilization Thermal activation Thermal stability |
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