| 铈对悬浮培养南方红豆杉细胞可溶性蛋白和紫杉醇合成动态的影响 |
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| 引用本文： | 李景川,马忠海,元英进,孙安慈,胡昌序.铈对悬浮培养南方红豆杉细胞可溶性蛋白和紫杉醇合成动态的影响[J].中国稀土学报,2001,19(2):162-166. |
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| 作者姓名： | 李景川 马忠海 元英进 孙安慈 胡昌序 |
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| 作者单位： | 1. 天津大学生化工程系,
2. 中国科学院植物研究所, |
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| 基金项目： | 国家教委“跨世纪优秀人才培养计划”基金项目；天津市科委重点攻关项目（963107211） |
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| 摘 要： | 研究Ce4 对悬浮培养南方红豆杉细胞可溶性蛋白和紫杉醇合成的动态影响，Ce4＋对可溶性蛋白合成和细胞活力的影响存在“分区和分叉现象，低浓度Ce4 (0.010mmol.L^-1)可显著提高可溶性蛋白的合成强度和细胞活力，高浓度Ce4 (5.00mmol.L^-1)对细胞表现为强伤害作用，适宜浓度Ce4 (1.00mmol.L^-1)在短期内可大幅度提高紫杉醇的合成速度，达到最高积累。
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| 关 键 词： | 稀土 铈 可溶性蛋白 紫杉醇 植物细胞培养 南方红豆杉 悬浮培养 细胞代谢 |
| 文章编号： | 1000－4343(2001)02－0162－05 |
| 修稿时间： | 1999年12月3日 |
Dynamic Effects of Cerium
on Synthesis of Soluble Protein and Taxol in Suspension Culture of Taxus Chinensis Var.
Maimei Cells |
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| LI Jing-chuan,Ma Zhong-hai,YUAN Ying-jin,SUN An-ci,HU Chang-xu.Dynamic Effects of Cerium
on Synthesis of Soluble Protein and Taxol in Suspension Culture of Taxus Chinensis Var.
Maimei Cells[J].Journal of the Chinese Rare Earth Society,2001,19(2):162-166. |
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| Authors: | LI Jing-chuan Ma Zhong-hai YUAN Ying-jin SUN An-ci HU Chang-xu |
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| Institution: | LI Jing chuan 1,MA Zhong hai 1,YUAN Ying jin 1,SUN An ci 2,HU Chang xu 2 |
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| Abstract: | The effects of Ce 4 on synthetic rule of soluble protein and Taxol in suspension culture of Taxus chinensis var. mairei were studied. The results show that the phenomenon of partition and bifurcation occurs in the effect of Ce 4 on the synthesis of soluble protein and cell activity. The synthetic strength of soluble protein and cell activity is improved by Ce 4 with low concentration and Ce 4 with high concentration is harmful to protein synthesis and cell activity. Ce 4 with certain concentration enhances Taxol synthesis. |
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| Keywords: | rare earths cerium soluble protein Taxol plant cell culture Taxus chinensis var mairei |
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