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Detection of environmental chemicals by SPR assay using branched cyclodextrin as sensor ligand |
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| Authors: | Kenjiro Hattori Tomoko Takeuchi Mika Ogata Ayumi Takanohashi Katsuhiko Mikuni Katsuyoshi Nakanishi Hideo Imata |
| Affiliation: | (1) Department of Nanochemistry, Faculty of Engineering, Tokyo Polytechnic University, Atsugi, Kanagawa 243-0297, Japan;(2) Bio-Research Corporation of Yokohama, 1-1-1 Fukuura, Kanazawa-ku, Yokohama, Kanagawa 236-0004, Japan;(3) Sagami Keisoku Co., 1892-1 Shimomizo, Sagamihara, Kanagawa 229-0015, Japan |
| Abstract: | We obtained the association constants Ka of estrogen (E2) and environmental chemicals by the surface plasmon resonance (SPR) assay using the immobilized mono-6-O-α-maltosyl-β-CD (G2βCD) compared with the immobilized β-CD and the immobilized estrogen receptor (ER). The association behavior of G2βCD was shown as a ER model compound. The calibration curve was determined by the initial rate of association depending on the various concentrations, and the minimum detectable concentrations in the order of parts per billion were calculated. The SPR assay has advantages that the pre-treatment of the sample is not necessary and the immobilized ligand is stable and useful for the repeated measurement. |
| Keywords: | Maltosyl cyclodextrin Surface plasmon resonance Environmental chemicals Estrogen receptor |
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