Reduction of allergenicity of irradiated ovalbumin in ovalbumin-allergic mice |
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Authors: | Ji-Hyun Seo Ju-Woon Lee Jae-Hun Kim Eui-Baek Byun Soo-Young Lee Il-Jun Kang Myung-Woo Byun |
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Affiliation: | aRadiation Application Research Division, Advanced Radiation Technology Institute, Jeongeup 580-185, Republic of Korea;bGraduate School of Biotechnology, Korea University, Yeongi 339-700, Republic of Korea;cDepartment of Pediatrics, Ajou University School of Medicine, Suwon 442-749, Republic of Korea;dDivision of Life Sciences, Hallym University, Chunchon 200-702, Republic of Korea |
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Abstract: | Egg allergy is one of the most serious of the immediate hypersensitivity reactions to foods. Such an allergic disorder is mediated by IgE antibodies stimulated by T-helper type 2 (Th2) lymphocytes. This study was undertaken to evaluate changes of allergenicity and cytokine profiles by exposure of irradiated ovalbumin (OVA), a major allergen of egg white, in the OVA-allergic mice model. OVA solutions (2 mg/ml in 0.01 M phosphate buffered saline (PBS) were gamma-irradiated to 50 and 100 kGy. The allergenicity in the OVA-allergy-induced mice model was remarkably reduced when challenged with irradiated OVA. Cultures of spleen cells harvested from OVA-sensitized mice showed a significant decrease in Th2 cytokine levels of ILs-4 and -5 with a concomitant increase in Th1 cytokine levels of IL-12 when co-cultured with irradiated OVA. However, IFN-γ level decreased dependant on the radiation dose of co-cultured OVA. The levels of IgEs and Th2-cytokine were reduced dependant on the radiation dose. These data show that the irradiated OVA could downregulate the activity of Th2 lymphocytes in OVA-sensitized mice. |
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Keywords: | Gamma irradiation Ovalbumin Allergy Therapeutics |
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