Highly sensitive spectrofluorimetric determination of trace amounts of lecithin |
| |
Authors: | Weiwei Bian Chongqiu Jiang |
| |
Affiliation: | (1) Department of Chemistry, Shandong Normal University, Jinan, 250014, China |
| |
Abstract: | A new spectrofluorimetric method was developed for the determination of trace amounts of lecithin using the ciprofloxacin (CIP)–terbium (Tb3+) ion complex as a fluorescent probe. In a buffer solution at pH=5.60, lecithin can remarkably reduce the fluorescence intensity of the CIP–Tb3+ complex at λ=545 nm. The reduced fluorescence intensity of the Tb3+ ion is proportional to the concentration of lecithin. Optimum conditions for the determination of lecithin were also investigated. The linear range and detection limit for the determination of lecithin were 1.0×10−6–3.0×10−5 mol L−1 and 3.44×10−7 mol L−1, respectively. This method is simple, practical, and relatively free of interference from coexisting substances. Furthermore, it has been successfully applied to assess lecithin in serum samples. |
| |
Keywords: | Lecithin Ciprofloxacin Spectrofluorimetry Terbium |
本文献已被 PubMed SpringerLink 等数据库收录! |
|