塑料芯片毛细管电泳电化学检测系统及其性能评价

近年来，高分子芯片毛细管电泳技术发展迅速，以聚甲基丙烯酸甲酯(PMMA)为代表的塑料电泳芯片由于其低廉的制作成本与良好的电渗性能，已经成为芯片电泳技术发展的一个重要方向，电化学检测具有灵敏度高、选择性好和易于微型化等优点，因此在塑料芯片电泳领域中具有较好的应用前景。

Configuration and Property Evaluation of a Novel Plastic Capillary Electrophoresis Microchip with Electrochemical Detection System

A poly(methyl methacrylate)-based capillary electrophoresis microchip, integrated with a microelectrode-based end-column amperometric detection, was described. A short unclosed portion of the separation channel from the detection cell was used as a working-electrode guide channel. With the aid of a three-dimension micropositioner and an optical microscope, a home-made 6 μm carbon-fiber microdisk electrode(tip diameter of 20 μm) was positioned along the guide channel at 30 μm distance from the channel outlet. With dopamine and catechol the as model analytes, the analytical performance of the system was demonstrated and the detection limit of 1.6×10-7mol/L for dopamine was achieved, to our knowledge, which is the lowest value than that from published literatures using electrophoresis microchip with end-column amperometric detection.