Optically-driven red blood cell rotor in linearly polarized laser tweezers |
| |
Authors: | Manas Khan Samarendra K Mohanty A K Sood |
| |
Institution: | (1) Department of Physics, Indian Institute of Science, 560 012 Bangalore, India;(2) Unit on Nanoscience and Technology-DST, Indian Institute of Science, 560 012 Bangalore, India;(3) Present address: Biomedical Applications Section, Centre for Advanced Technology, 452 013 Indore, India |
| |
Abstract: | We have constructed a dual trap optical tweezers set-up around an inverted microscope where both the traps can be independently
controlled and manipulated in all the three dimensions. Here we report our observations on rotation of red blood cells (RBCs)
in a linearly polarized optical trap. Red blood cells deform and become twisted in hypertonic phosphate buffer saline and
when trapped, experience an unbalanced radiation pressure force. The torque generated from the unbalanced force causes the
trapped RBC to rotate. Addition of Ca++ ions in the solution, keeping the osmolarity same, makes the cell membranes stiffer and the cells deform less. Thus the speed
of rotation of the red blood cells can be controlled, as less deformation and in turn less asymmetry in shape produces less
torque under the radiation pressure resulting in slower rotation at the same laser power |
| |
Keywords: | Rotation of red blood cell optical tweezers dual optical trap |
本文献已被 SpringerLink 等数据库收录! |
|