| 尼龙亲和膜色谱法纯化半胱氨酸蛋白酶抑制剂 |
| |
| 引用本文： | 苏赛男,聂华丽,朱利民,陈天翔.尼龙亲和膜色谱法纯化半胱氨酸蛋白酶抑制剂[J].分析化学,2008,36(2):231-234. |
| |
| 作者姓名： | 苏赛男 聂华丽 朱利民 陈天翔 |
| |
| 作者单位： | 东华大学生物科学与技术研究所,上海,201620 |
| |
| 基金项目： | 国家自然科学基金资助项目(No.50773009) |
| |
| 摘 要： | 建立了一种快速、简便的分离半胱氨酸蛋白酶抑制剂的方法,尼龙膜经壳聚糖改性后,以木瓜蛋白酶为配基制备了一种新型的蛋白质分离材料,并研究了此分离材料的物理及化学性能。所得到的亲和膜上固定化木瓜蛋白酶的热稳定性提高,最适催化温度为55℃,最适pH值为8.0,米氏常数为353.8 g/L;将该亲和膜应用于半胱氨酸蛋白酶抑制剂的分离纯化,在对洗脱液、洗脱速度进行优化的基础上,成功的分离纯化出半胱氨酸蛋白酶抑制剂,纯化倍数为7.25倍。
|
| 关 键 词： | 尼龙膜 木瓜蛋白酶 半胱氨酸蛋白酶抑制 |
| 收稿时间： | 2007-07-11 |
| 修稿时间： | 2007-08-17 |
Purification of Cystatin by Nylon Membrane Affinity Chromatography |
| |
| Su Sai-Nan,Nie Hua-Li,Zhu Li-Min,Chen Tian-Xiang.Purification of Cystatin by Nylon Membrane Affinity Chromatography[J].Chinese Journal of Analytical Chemistry,2008,36(2):231-234. |
| |
| Authors: | Su Sai-Nan Nie Hua-Li Zhu Li-Min Chen Tian-Xiang |
| |
| Abstract: | In order to establish a novel method for the purification of cystatin,nylon membranes are first treated with chitosan before being used as the affinity carrier.Papain as a ligand has been immobilized on the actived membranes with glutaraldehyde as crosslinking agent.The physical and chemical properties of the matrix are studied.The thermo stability of the immobilized papain on nylon membrane is improved.In pH 8.0,the Km of immobilized papain is 353.8 g/L.After optimization of experimental conditions,such as elution solution and elution-rate,the cystatin is successfully purified from the potato juice.The purify ratio is 7.25. |
| |
| Keywords: | Nylon membrane papain cystatin |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
