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Novel core-shell nanoparticles and their application in high-capacity immobilization of enzymes |
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| Authors: | Xuepu Mao Jinfeng Huang Man Fai Leung Zhiyun Du Lin Ma Zhishu Huang Pei Li Lianquan Gu |
| Institution: | (1) School of Pharmaceutical Sciences, Sun Yat-sen University, 510080 Guangzhou, P.R. China;(2) School of Chemistry and Chemical Engineering, Sun Yat-sen University, 510275 Guangzhou, P.R. China;(3) Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, P.R. China |
| Abstract: | Novel core-shell nanoparticles consisting of poly(methyl methacrylate) (PMMA) cores coated with synthetic polymer and biopolymer (polyethyleneimine, chitosan, and casein) shells were synthesized via direct graft copolymerization of methyl methacry late from hydrophilic polymers in the absence of surfactant. Average hydrodynamic diameters of the nanoparticles ranged from 163 to 263 nm. High-capacity (up to 530 mg/g) immobilizations of enzymes and high-activity retained percentage (E spe) (up to 90%) were achieved. |
| Keywords: | Core shell poly(methyl methacrylate) nanoparticles immobilized cellulase |
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