硝基磺酚C光度法测定蛋白质的研究

蛋白质的定量分析是生化研究、临床化验和食品检验等领域经常涉及的内容.以有机小分子作光谱探针测定蛋白质,如甲基橙^1,2]、考马斯亮蓝G-250^3]、溴酚蓝^4]、溴甲酚绿^5]和偶氮胂Ⅲ^6]等已得到研究.

Spectrophotometric Determination of Proteins by Using Nitrosulfophenol C

In this paper, it is found firstly that the nitrosulfophenol C can react with proteins to form a pure blue complex in BrittonRobinson buffer solution at pH 34 and room temperature, which gives maximum absorption peak at 675 nm with 100 nm to red shift compared to that of nitrosulfophenol C. According to studies on optimum conditions, a new spectrophotometric method for determination of proteins was established. The standard working curves of eight kinds of proteins with larger linear ranges and higher sensitivity were determined. The new method was successfully applied to the determination of protein in human serum samples with satisfactory results in agreement with those of the classical coomassie brilliant blue G250(CBB G250) method.