Phosphorylation state-responsive lanthanide peptide conjugates: a luminescence switch based on reversible complex reorganization |
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Authors: | Tremblay Matthew S Zhu Qing Martí Angel A Dyer Joanne Halim Marlin Jockusch Steffen Turro Nicholas J Sames Dalibor |
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Institution: | Department of Chemistry, Columbia University, New York, New York 10027, USA. |
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Abstract: | reaction: see text] A luminogenic probe for peptide dephosphorylation has been developed. It consists of a serine-/tyrosine-containing peptide modified on the N-terminus with a tryptophan residue and a DTPA chelate capable of binding Tb(3+). We propose a mechanistic model for the luminescence enhancement based on the interconversion of monomeric and dimeric lanthanide species, which is affected by the phosphorylation state of the serine or tyrosine residue. The optical switch reports effectively on phosphatase-catalyzed dephosphorylation in vitro. |
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