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Microbore columns in quantitative column liquid chromatography |
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| Authors: | J H M van den Berg H W M Horsels R J M Groenen |
| Institution: | (1) Analytical Department, DSM Research and Patents, PO Box 18, 6160 MD Geleen, The Netherlands;(2) Present address: Fundamental Polymer Research Department, DSM Research and Patents, PO Box 18, 6160 MD Geleen, The Netherlands;(3) Present address: Trainee of the Zuidlimburgse Laboratoriumschool Sittard, Montgomerystraat 7, 6135 BW Sittard, The Netherlands |
| Abstract: | Summary Some quantitative implications of the use of microbore columns in column liquid chromatography are investigated. Although for several pumps the flow rate stability at 50  l min–1 is slightly worse than that of pumps operating at 1 ml min–1, the quantitative performance with respect to repeatability and reproducibility of response factors equals that of conventional liquid chromatography. Thermostatting is strongly recommended for microbore column operation. |
| Keywords: | Column liquid chromatography Microbore column Flow rate stability Performance Quantitative analysis |
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